Thin Layer Chromatography

Silica gel with calcium sulphate (CaSO4 ½ H2O) as binder is by far the most extensively used adsorbent in Thin Layer Chromatography because of the excellent separation it affords. When coated on a plate in the form of thin layer it serves as a versatile solid stationary phase for adsorption and partition chromatography.

Range of products in Thin Layer Chromatography
a Silica Gel TLC `H’ without binder
b Silica Gel HF – 254 with fluorescent indicator F – 254
c Silica Gel TLC `G’ with binder i.e. CaSO4 ½ H2O
d Silica Gel GF – 254 with fluorescent indicator F – 254

APPLICATIONS

Silica Gel – `G’ can be successfully employed in both qualitative and quantitative analysis of alkaloids, amino acids, oils and fats, vitamins, plant pigments, drugs and sugars.

Specification of Silica Gel – Thin Layer Chromatography
    Sr. No.  Properties   Unit  Grade
1   Grades H HF – 254   G GF – 254
2 PH value (10% water Suspension) 7 ± 0.5 7 ± 0.5 7 ± 0.5 7 ± 0.5
3   Iron, as Fe   ppm, max. 300 200 300 200
4   Chloride, as Cl   ppm, max.   300 200 300 200
5   CaSO4 0.5 H2O   %   —- 13.0 ± 0.5 13.0 ± 0.5
6 Suitability Test (Plate)   —-   Passes Passes Passes Passes

PACKAGING FOR SILICA GEL - TLC GRADE

FD – Fiber Drum – 25KG
C – Corrugated Box – 5 & 10 KG