SILICA GEL FOR COLUMN CHROMATOGRAPHY

Column Chromatography is today one of the most important and widely used techniques for the separation and analysis of complex organic mixtures.

Silica gel for column chromatography are available in any desired particle size for pilot plant or plant applications.

Silica Gel Column Chromatography

Silica gel used in column chromatography is the ideal method for purification and separation. It is a technique in which the stationary phase is silica gel and activated alumina powder and the mobile phase is a liquid. The principle of active compound separation depends on the activity of adsorbents like silica gel and the polarity of the solvent. Solvent polarity should be very low, and silica activity should be high in column chromatography in order to successfully separate compounds.

For top-tier silica gel column chromatography manufacturers, turn to Desicca Chemicals Pvt Ltd. We are the foremost industry leaders committed to elevating your chromatographic separations. Our comprehensive product range encompasses a variety of 230/400 particle sizes and pore diameters, addressing your diverse separation requirements effectively.

Conversely, if the solvent’s polarity registers as exceedingly high and the activity of the silica gel used in column chromatography remains elevated, it yields unsatisfactory results in compound separation. This translates to impurities lingering even after the purification and isolation processes.

 

Procedure of Column Chromatography Using Silica Gel

 

Chemical analysis relies heavily on column chromatography using silica gel for the separation of complex mixtures, making it the most common separator technique. This technology relies on the principles of chromatography to separate chemical compounds within a mixture by exploiting the interaction between two phases: the stationary phase and the mobile phase.

 

  • Analytical Chromatography: Analytical Chromatography, the initial phase of this process, typically employs small amounts of silica gel mesh sized 60-120. Analytical column packaging allows for the precise analysis of the mixture’s composition and purification percentage. This method is not only simple but also cost-effective, making it a favored choice in analytical chemistry for solving chemical substances using thin-layer plates coated with silica gel on a glass plate. It has become a standard analytical tool in pharmaceutical laboratories.

 

  • Preparative Chromatography: Preparative Chromatography, on the other hand, focuses on optimizing chromatography technologies for larger-scale separations. After the preparative process, which may involve semi-preparative chromatography, improvements are made to the impurity profile and the speed of separation.

 

  • Process Chromatography:  Process Chromatography is essential for large-scale applications, particularly in the purification of biologics such as proteins, viruses, vitamins, hormones, and antibodies. In this context, stationary phase adsorbents like silica gel and activated alumina are used in bulk quantities, with silica gel mesh size for column chromatography ranging from 100 /200 and 230 /400. This ensures a high degree of purity in the isolated active compounds.

Gravity Chromatography: Gravity Chromatography is a manual process that requires continuous monitoring. It relies on gravitational force to allow the solvent to flow down the column at a rate that can be manually controlled. Practical silica gel mesh sizes, such as 70/325 and 60/200 mesh microns, are commonly employed in this technique.

 

Column Chromatography using silica gel is a versatile and essential method for the separation and purification of complex mixtures. By carefully selecting the silica gel mesh size for column chromatography and optimizing the process conditions, scientists can achieve precise results in both analytical and preparative chromatography, making it an invaluable tool in various scientific fields. The use of silica gel in column chromatography is fundamental to its success and wide-ranging applications.

MESH SIZES MANUFACTURED BY US
a 60 / 120 mesh
b 100 / 200 mesh
c 60 / 200 mesh. (equivalent to Davision 62)
d 230 / 400 mesh
e 70 / 325 mesh
f 30 / 200 mesh. (equivalent to Davision 12)
SPECIFICATION FOR SILICA GEL – COLUMAN CHROMATOGRAPHY 
Sr. No. Properties Unit Llimit
1 pH value (10% water Suspension)   —-   7.0 ± 0.5
2 Chloride as Cl   ppm, max.   200
3 Iron as Fe   ppm, max.   200
4 Activity, Brockmann   I – II
5 First Mesh, Coarser
Second Mesh, Finer
% max.

% max.

5

10

APPLICATIONS of Silica Gel Column Chromatography

Silica Gel Column Chromatography finds wide-ranging applications across various industries:

 

  • Water soluble alkaloids
  • Separation of Ceratridene alkaloids
  • Isolation of pyrrolizidine alkaloids
  • Separation of clavinet alkaloids from Ipomoea Muricata on a large scale
  • Preparative isolation & purification
  • Purification of quaternary ammonium & pyridinium compounds
  • Macrolide antibiotics
  • Ranitidine dimer separation
  • Losartan potassium
  • Enzyme high-performance liquid chromatography for food applications at large scales
  • Purification of peptides
  • Amino acid purification
  • Proteins purification
  • Separation of Resorcinol and catechol

NOTE

For 230 / 400 particle size :
First mesh Coarser – 10% max.
Second mesh Finer – 15% max.

PACKAGING FOR SILICA GEL - C.C Grade

Fiber Drum – 25KG

At Desicca Chemicals Pvt Ltd, we are dedicated to providing you with Premium Silica Gel Column Chromatography Manufacturers that empower your chromatographic separations. With our diverse range of 230/400 Particle Sizes and pore diameters, we ensure that your specific requirements are met, and your chromatographic processes are optimized.

For inquiries, product information, or custom solutions, please don’t hesitate to contact us at salesdesiccachemicals@gmail.com, desiccachemicals@gmail.com or exportsdesicca@gmail.com. Our team of experts is ready to assist you in achieving unparalleled results in your chromatography endeavors. Trust us for all your Silica Gel Column Chromatography needs.

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