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Thin Layer Chromatography

 

Silica gel with calcium sulphate (CaSO4 H2O) as binder is by far the most extensively used adsorbent in Thin Layer Chromatography because of the excellent separation it affords. When coated on a plate in the form of thin layer it serves as a versatile solid stationary phase for adsorption and partition chromatography.

 

Range of products in Thin Layer Chromatography

a

Silica Gel TLC `H without binder

b

Silica Gel HF - 254 with fluorescent indicator F 254

c

Silica Gel TLC `G with binder i.e. CaSO4 H2O

d

Silica Gel GF 254 with fluorescent indicator F 254

 
APPLICATIONS
 

Silica Gel - `G can be successfully employed in both qualitative and quantitative analysis of alkaloids, amino acids, oils and fats, vitamins, plant pigments, drugs and sugars.

 

Specification of Silica Gel Thin Layer Chromatography

Sr. No.

Properties

Unit

Grade

1

Grades

 

H

HF 254

G

GF 254

2

PH value (10% water Suspension)

 

7 0.5

7 0.5

7 0.5

7 0.5

3

Iron, as Fe

ppm, max.

300

200

300

200

4

Chloride, as Cl

ppm, max.

300

200

300

200

5

CaSO4 0.5 H2O

%

----

---

13.0 0.5

13.0 0.5

6

Suitability Test (Plate)

----

Passes

Passes

Passes

Passes

 
Packaging for Silica Gel - TLC Grade

 

FD Fiber Drum - 25KG
 
C Corrugated Box - 5 & 10 KG
 
 

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